聚天冬酰胺衍生物对P(MMA-GMA-DVB)微球的修饰

Modification of P(MMA-GMA-DVB) Microspheres by Polyasparagine Derivatives

用无皂乳液聚合法合成了不同交联剂含量(单体的20～50wt%)的表面带环氧基团的窄分散的P(MMA-GMA-DVB)(PMGD)复合微球,以L-天冬门酸为原料用磷酸催化溶剂法制备了聚琥珀酰亚胺(PSI),以N,N-二甲基甲酰胺为溶剂将PSI接枝到PMGD微球表面.元素分析结果证明,当微球中DVB的含量为40%时,微球表面修饰的PSI量最多.然后用乙二胺对微球表面剩余的PSI环进行开环,利用微球表面的氨基键合荧光素异硫氰酸荧光素(FITC),得到了荧光微球.

Narrow-dispersed P（MMA-GMA-DVB） （PMGD） microspheres with different amout of DVB from 20 to 50 weight percentages were prepared by soap-free emulsion polymerization and polysuccinimide （PSI） was prepared by polycondensation of L-aspartic acid （L-ASP） using phosphoric acid as a catalyst in sulfolane/mesitylene solvent.Then,PSI was attached to the surface of PMGD microspheres containing different amount of DVB in N,N-dimethylformamide solvent.The elemental analysis results showed that the largest amount of PSI was attached to the surface of P（MMA-GMA-DVB） microspheres when the weight content of DVB was 40%.Then the remains of active rings of the PSI on the surface of microspheres were opened by ethanediamine,at last,fluorescent microspheres were achieved by reacting with fluorescein isothiocyanate （FITC）.