固定化荧光假单胞菌脂肪酶用于催化低酸值餐饮废油的酯交换反应

Transesterification of Low Acid Value Waste Oil from Restaurant Using Immobilized Pseudomonas fluorescens Lipase

动植物油脂的酯交换反应是制备脂肪酸烷基酯的一个重要反应。其中,脂肪酸甲酯、乙酯为普通石化柴油的优质替代燃料。脂肪酶易于固定在海于连续生产生物柴油,试验研究了一株商业荧光假单胞菌(P.fluorescens ATCC 13525)固定化脂肪酶催化低酸值餐饮废油和甲醇合成生物柴油的最优工艺条件。通过对反应温度、pH值、醇油物质的量比、酶的用量,反应时间等重要参数的研究,得到最优工艺条件:当反应温度为40℃,pH 7.0,醇油物质的量比4∶1,酶用量为3.0 g,反应时间48 h,反应体系含水量为8%时,生物柴油的得率最高。所制备的产品经分析测试,完全可以替代普通石化柴油,而且对现有内燃机无需做任何改动。

Transesterification of vegetable/animal oils is an important reaction to produce methyl and ethyl esters as substitutes for diesel fuel.P.fluorescens lipase is easily immobilized within sodium alginate for continuous production.The optimum conditions of synthesis of biodiesel from low acid value（LAV） restaurant waste oil and methanol catalyzed by a commercial immobilized lipase from P.fluorescens ATCC 13525 have been studied.The important parameters like reaction temperature,pH,methanol/oil molar ratio,enzyme dosage and reaction time were studied.Results： The maximum yield of biodiesel can be obtained at the optimum conditions of molar ratio 4∶ 1,water content 8 %,enzyme dosage 3.0 g,pH 7.0,reaction temperature 40 ℃,and reaction time 48 h.The products were compared with conventional petroleum based diesel and it was found that the product could be used as an effective alternate fuel in existing diesel engine without any hardware engine modification.