氧离子辐射对体外人精子自发化学发光、运动性、顶体反应和存活率的影响(英文)

EFFECTS OF ^(16)O^(+6) ION IRRADIATION ON HUMAN SPERM SPONTANEOUS CHEMILUMINESCENCE, MOTILITY, ACROSOME REACTION AND VIABILITY IN VITRO

我们研究了不同剂量(0,0.25,0.5,1,2,4,8,16,32,64Gy)3.17MeV/u氧离子对体外人精子自发化学发光(SCL)、运动性,顶体反应(AR)和存活率的影响。结果表明,精子SCL随辐射剂量明显增强,最低有效剂量为0.5Gy;精子运动性在0.5—2Gy,AR在0.5—4Gy辐射组均明显高于其对照组,但均随辐射剂量的进一步增加而明显降低;在0.25—8Gy剂量之间,精子存活率与其对照组相比无明显变化,但当剂量进一步增加至16—64Gy时,存活率急剧下降。这些结果提示,低剂量重离子辐射能对精子运动性及AR等功能产生兴奋效应。而大剂量则明显抑制这些功能,甚至造成精子死亡。其作用机制可能与重离子辐射诱导的自由基反应有关。

Effects of 16O+6 ion irradiation with different doses on human sperm spontaneous chemilu-minescence (SCL), motility, acrosome reaction (AR) and viability were examined. Spermatozoa were irradiated with 0, 0. 25, 0. 5, 1, 2, 4, 8, 16, 32, or 64 Gy 16O+6 ion beam at the energy of 3. 17 MeV/u. After irradiation, samples were analyzed by SCL measurement at 1, 2 and 3 h of incubation; motility was determined by the transmembrane migration method within 2 h of incubation; the percentage of AR and viability was evaluated by the triple-stain technique at 3. 5 h of incubation. The results showed; sperm SCL was significantly increased with irradiation doses and the lowest effective dose was 0. 5 Gy; compared with controls, the transmembrane migra- tion ratio of spermatozoa progressively elevated with irradiation doses at 0. 5, 1, and 2 Gy; the percentage of sperm AR markedly increased in 0. 5 - 4 Gy irradiation and the optimal dose was 2 Gy, and then significant decreased with further increase of irradiation doses; the viability had no significant change within 0. 25 - 8 Gy, but was progressively decreased at 16, 32 and 64 Gy. These data suggested that heavy ion at low doses increased motility and AR, whereas had deleterious effects at higher doses, which are associated with free radical reactions induced by heavy ion irradiation.