栀子中熊果酸的含量测定

目的 :高效液相色谱法测定栀子中熊果酸含量。方法:采用高效液相色谱法,以熊果酸为化学对照品,固定相:SymmitryC_(18)键合硅胶柱(4.6mm×250mm,5μm),流动相:甲醇:水:冰醋酸:三乙胺(90:10:0.03:0.06),流速0.6ml/min,温度25℃,波长210nm测定栀子中熊果酸含量。结果 :熊果酸在0.1～3.0μg范围内峰面积A与其质量M呈现良好的线性关系,其回归方程为:A=-12954.8829+452850.9603M,r=0.9996,精密度实验RSD=1.585%,仪器精密度良好;稳定性实验RSD=1.953%,在3.5h测定熊果酸的含量是稳定的:重现性实验RSD=2.150%,平均回收率100.4%。结论 :高效液相色谱法适合栀子中熊果酸的含量测定,该方法操作简便,结果稳定,精密度、重现性、回收率较好,值得推广。

ObjectiveTo establish the method of determining the cotent of ursolic acid in Fructus Gardeniae by HPLC.oMethodsHPLC was used to determine the content of Ursolic acid in Fructus Gardenice ursolic acid as the standard, Solid phase： SymmitryC：s （4.6mm×250mm,5μm）, Liquid phase：Methyiaicohol：Water：Acetic acid：Triethylaminum （90：10：0.03：0.06）, Flow rate：0.6ml·min^-1 ,temperature：25℃,wavelength：210 nm.ResultsThe calibration curve was linear in the range of 0.1 -3.0μg, and the return formula was A=-12954.8829＋452850.9603＊M, r= 0.9996,RSD=1.585%,stablity RSD%=1.953 ,repeatability RSD=2.150% ,recoveryratewas 100.4%.Conclusion HPLC is suitable for the determi- nation of ursolic acid in Fructus Gardeniae. This method is simple, the result is stable and has good repeatability,recovery and precision. It can be widely used for the quality control of Fructus Gardeniae.