真空渗透法转化植物的研究

IN PLANTA TRANSFERMATION BY VACCUM INFILTRATION

以拟南芥为材料介绍一种不需组培的原位植物转化方法—真空渗透法。将含有Ｔ—ＤＮＡ载体的农杆菌细胞悬液，用真空渗透的方式转化愈伤的完整植株，从而直接获得转化的种子。该法快速简便、重复性好，不需经过组培阶段即可获得转化植株。

Adrian J. Culter (Plant Biotechnology Institute, National Research Council of Canada, Canada)A method for in situ transformation, vaccum infiltration is presented. The approach is vaccum infiltration of a suspension of Agrobacterium cells containing T-DNA vector into Arabidopsis thaliana; thereby the transformed seeds can be harvested directly. This reproducible, non-tissue cultural procedure yields stably transformed A. thaliana seeds and its transformation frequency is high enough for introducing foreign genes and characterizing their expression.