摘要
以５－氟脲嘧啶（５－ｆｌｕｏｒｏｕｒａｃｉｌ，５－Ｆｕ）腹腔注射治疗了１２只携有金属硫蛋白（Ｍｅｔａｌｏｔｈｉｏｎｅｉｎ，ＭＴ）启动子和增强子序列、ｂｃｒ－ａｂｌｃＤＮＡ（ｐ２１０）序列及ＳＶ４０剪切和Ｐｏｌｙ（Ａ）信号序列的转基因慢粒白血病（ｃｈｒｏｎｉｃｍｙｅｌｏｉｄｌｅｕｋｅｍｉ－ａ，ＣＭＬ）ＭＴ／ｐ２１０ｂｃｒ－ａｂｌ小鼠，于治疗的第５ｄ及第１０ｄ从不同脏器提取总ＲＮＡ、ＤＮＡ及蛋白质，分别进行ＰＣＲ分析、ＲＴ－ＰＣＲ检测被转移基因的表达水平及免疫沉淀法分析ｐ２１０ｂｃｒ－ａｂｌ转基因产物的激酶活性．结果表明，被转移基因在脾脏和骨髓中表达水平较高，胸腺和肾脏中呈低水平表达．５－Ｆｕ活体治疗１０ｄ后。
Abstract CML (chronic myeloid leukemia) transgenic mice carrying metallothionein(MT) promoter/enhancer, bcr abl(p210) cDNA and SV40 splicing/poly(A) signal sequences were treated by intraperitoneal injection of 5 Fu(5 fluorouracil). DNA, total RNA and protein were isolated and purified from different organs of the mice after the 5 Fu treatment for 5 days and 10 days respectively. The transgene and its expression in the
出处
《生命科学研究》
CAS
CSCD
1999年第1期36-41,共6页
Life Science Research
基金
国家自然科学基金
