摘要
用胸腺素免疫新西兰兔，获得抗胸腺素抗体，建立了胸腺素ＥＬＩＳＡ定量测定法。该法检测灵敏度为３．２ｎｇ／ｍｌ，批内和批间变异系数分别为４．４％和８．９％，方法特异性鉴定显示与另外７种激素无明显交叉反应。该方法灵敏度高、专一性强、重复性好，可用于胸腺素产品的质量检测。
We established an enzyme linked immunosorbent assay (ELISA) to measure thymosin. The antibody to thymosin was prepared in rabbits. The sensitivity of ELISA was about 3.2ng/ml, intra and interassay coefficient of variation was 4.4% and 8.9%, respectively. It didn't significantly cross react with other seven hormones. The method was sensitive, specific and reproducible. It can be used to measure content of thymosin production and has great application value.
出处
《氨基酸和生物资源》
CAS
1999年第1期21-23,共3页
Amino Acids & Biotic Resources
