淋巴细胞凋亡与p53蛋白表达关系的研究

Study of the Relation on Apoptosis and p53 Expression in Lymphocytes.

培养Ｂ９５８细胞，分离ＥＢ病毒，转染外周血和扁桃体淋巴细胞，建立永生化的ＬＣＬｓ和ＴＬＣＬ细胞株；带有ｗｔＰ５３基因的ＬＣＬｓ在ＤＮＡ损伤剂———顺铂处理前未检出ｐ５３蛋白，经顺铂处理后，ＬＣＬｓ随作用时间延长细胞存活率明显下降、ｐ５３蛋白水平升高、ＤＮＡ电泳显出梯状带；含ｍｔＰ５３基因的淋巴瘤细胞在顺铂处理前可检出高浓度的ｐ５３蛋白，经顺铂处理后，细胞存活率与ｐ５３蛋白并无明显改变．这些结果表明：顺铂引起细胞ＤＮＡ损伤、激活ｗｔｐ５３蛋白的表达。

Epatein Barr Virus(EBV) is isolated from B95 8 cell line, the primary B lymphocytes from human blood and tonsil tissue are transfected with EBV in order to gain immortalized lymphoblastoid cell lines(LCLs) and tonsil lymphoblastoid cell lines(TLCL). The LCLs and TLCL contain wt p53 gene, and do not express p53 proteins before treatment with cisplatin. Cell viability are decreased, p53 proteins are accumulated,and DNA ladders appear in LCLs and TLCL with time extension after treating with 10 mg/L. Lymphoma cell lines(BJAB, Raji, Ramos, Molt4) carring mutant p53 gene show accumulation of p53 protein before treatment with cisplatin. In paralleled experiment, the lymphoma cell lines appear relatively resistant to the doses of cisplatin. The results suggest that cisplatin cause DNA damage of LCLs and TLCL, activate wt p53 expression, and cells with DNA damage enter the pathway of apoptosis induced by wt p53.