一种用分光光度计检测氧自由基的新方法

A Spectrophotometer Method Testing Oxygen Radicals .

报告检测过硫酸铵／Ｎ，Ｎ，Ｎ′，Ｎ′四甲基乙二胺体系所产生的氧自由基的新方法．Ｏ·２与羟胺溶液反应生成ＮＯ－２，ＮＯ－２经对氨基苯磺酸和α萘胺显色在波长５３０ｎｍ处有专一吸收峰，其颜色深浅与产生的Ｏ·２呈量效关系．

A spectrophotometer method was used to test oxygen radicals produced by a system of ammonium persulphate and tetramethylethylenediamine(AP TEMED).The reaction of hydroxylamine and O · 2 produced NO - 2 and then developed by sulphanilic acid and α naphthylamine.The results indicated that there was a special absorption at 530 nm when the sample was tested by spectrophotometer, and its color responsiveness showed a quantitative relation to O · 2.Otherwise, ascorbic acid as a matter deleted oxygen radicals had a significantly quantitative relation too.