高寒藏药——抱茎獐牙菜组织培养研究及其愈伤组织有效成分的测定

TISSUE CULTURE AND ANTIHEPATITIS CONSTITUENT IN CALLI OF SWERTIA FRANCHETIANA H. SMITH

从抱茎獐牙菜的胚轴、幼叶及未成熟种子诱导出愈伤组织并再生植株，试验选用ＭＳ、Ｂ５和Ｎ６三种培养基，其中以附加２．４－Ｄ３．０ｍｇ／Ｌ＋６－ＢＡ０．５ｍｇ／Ｌ的ＭＳ培养基诱导率最高；以附加６－ＢＡ０．５ｍｇ／Ｌ＋ＮＡＡ０．２ｍｇ／Ｌ的ＭＳ培养基分化苗频率最高；以附加２．４－Ｄ２．０ｍｇ／Ｌ＋６－ＢＡ０．５ｍｇ／Ｌ的ＭＳ培养基愈伤组织的生长最好。结果表明，外植体，培养基，激素等对愈伤组织诱导、继代和分化均有明显影响。采用高压液相色谱法（ＨＰＬＣ）测定抱茎獐牙菜愈伤组织中齐墩果酸含量的结果表明，愈伤组织中齐果墩酸含量因培养基。

Calli and the regenerative plants were induced from young leaves, immature seeds and hypocotyl of Swertia franchetiana H. Smith. Three kinds of media, MS, B 5 and N 6 were set up in the experiment. Calli were established with the highest frequency onto MS medeium containing 3mg/L 2,4-D and 0.5mg/L 6BA. The regenerative plants are obtained with the highest frequency onto MS medeium, Containing 0.5mg/L 6-BA and 0.2mg/L NAA. The induction and maintenence and differentiation of the calli were affected markly by the basic media and compound 2,4-D and 6-BA in the media etc. The Oleanolic acid content in calli of Swertia franchetiana H. Smith were determined by HPLC method.