新三黄片中黄芩苷在大鼠体内的药物动力学研究

STUDY ON THE PHARMACOKINETICS OF BAICALIN IN NEW SAN-HUANG TABLET IN RATS

目的:建立新三黄片中黄芩苷在大鼠体内的药物动力学研究方法。方法:以雄性Wistar大鼠为实验动物,按照1650mg/kg(相当于黄芩苷200mg/kg)的剂量灌胃给予新三黄片,于给药后不同时间点取血,应用RP-HPLC方法测定大鼠血浆中黄芩苷的含量。应用3p97药动学软件处理血药浓度数据,拟合新三黄片中黄芩苷在大鼠体内的吸收模型,获得药动学参数。结果:黄芩苷的线性范围为0.32μg/ml-6.4μg/ml,在大鼠体内呈二室吸收模型,主要的药物动力学参数为T1/2(α)272min、T1/2(β)353min、T1/2(ka)102.1min、AUC 1431.9μg.min/ml、V 584ml、CL(s)1.15 L/min。结论:本实验建立的HPLC测定黄芩苷血药浓度的方法,灵敏度高,专属性强,易操作,结果准确可靠,可用于新三黄片中黄芩苷的药物动力学研究。

Objective：To establish research methods on pharmacokinetics in vivo of baicalin in new San- huang tablet in rats.Methods：Mate Wistar rats were lavaged with new San-huang tablets in dose of 1650mg/ kg （equivalent 200mg/kg baicalin）. RP-HPLC was used to determine the baicalin content in rats＇ plasma at different time after administration. 3p97 software was used to deal with data of medicine concentration in plasma and calculate pharmacokinetic parameter for baicalin in rats.Results：The linear range of baicalin was 0.32μg/ml-6.41μg/ml. The absorption of baicalin in San-huang tablets in rats consistent with two-compartment model. The pharmacokinetic parameters of baicalin were T1/2（α）272min, T1/2（β）353min, T1/2（ka）102.1min, AUC 1431.9μg.min/ml, V 584ml and CL（s）1. 15.Conelusions：The method is simple, sensitive and operated easily. It can be used to study the pharmacokinetic of baicalin in new San-huang tablet.