超高效液相色谱-串联质谱法同时测定食用油中痕量的δ-9-四氢大麻酚、大麻酚和大麻二酚

Simultaneous determination of δ-9-tetrahydrocannabinol,cannabidiol and cannabinol in edible oil using ultra performance liquid chromatography-tandem mass spectrometry

建立了超高效液相色谱-串联质谱(UPLC-MS/MS)同时测定食用油中δ-9-四氢大麻酚(THC)、大麻酚(CBN)和大麻二酚(CBD)的方法。目标分析物经甲醇提取、中性氧化铝固相萃取柱净化后,采用UPLC-MS/MS分离和检测。实验以氘代四氢大麻酚(THC-D3)为内标物,采用同位素内标法定量。在3个添加水平下,目标物的平均回收率为68.0%～101.6%,相对标准偏差为7.0%～20.1%。方法检出限为0.06～0.17μg/kg,定量限为0.20～0.52μg/kg。该方法能够满足食用油中痕量四氢大麻酚、大麻酚和大麻二酚检测的需要。

A method for the simultaneous determination ofδ-9-tetrahydrocannabinol（THC）,cannabidiol（CBD）and cannabinol（CBN）in edible oil was developed using ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS）.The target compounds were extracted with methanol,purified by an LC-Alumina-N solid phase extraction cartridge,separated and detected by the UPLC-MS/MS.Quantitative analysis was corrected by an isotope internal standard method usingδ-9-THC-D3as internal standard.Average recoveries for the target compounds varied from68.0%to 101.6% with the relative standard deviations ranging from7.0%to 20.1% at three spiked levels.The limits of detection（LOD）of the method were from0.06-0.17μg/kg and the limits of quantification（LOQ）were in the range of 0.20-0.52μg/kg.The results showed that the method is able to meet the requirements for the simultaneous determination of THC,CBD and CBN in edible oil.