摘要
建立了超高效液相色谱-串联质谱(UPLC-MS/MS)同时测定食用油中δ-9-四氢大麻酚(THC)、大麻酚(CBN)和大麻二酚(CBD)的方法。目标分析物经甲醇提取、中性氧化铝固相萃取柱净化后,采用UPLC-MS/MS分离和检测。实验以氘代四氢大麻酚(THC-D3)为内标物,采用同位素内标法定量。在3个添加水平下,目标物的平均回收率为68.0%~101.6%,相对标准偏差为7.0%~20.1%。方法检出限为0.06~0.17μg/kg,定量限为0.20~0.52μg/kg。该方法能够满足食用油中痕量四氢大麻酚、大麻酚和大麻二酚检测的需要。
A method for the simultaneous determination ofδ-9-tetrahydrocannabinol(THC),cannabidiol(CBD)and cannabinol(CBN)in edible oil was developed using ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The target compounds were extracted with methanol,purified by an LC-Alumina-N solid phase extraction cartridge,separated and detected by the UPLC-MS/MS.Quantitative analysis was corrected by an isotope internal standard method usingδ-9-THC-D3as internal standard.Average recoveries for the target compounds varied from68.0%to 101.6% with the relative standard deviations ranging from7.0%to 20.1% at three spiked levels.The limits of detection(LOD)of the method were from0.06-0.17μg/kg and the limits of quantification(LOQ)were in the range of 0.20-0.52μg/kg.The results showed that the method is able to meet the requirements for the simultaneous determination of THC,CBD and CBN in edible oil.
出处
《色谱》
CAS
CSCD
北大核心
2010年第11期1015-1019,共5页
Chinese Journal of Chromatography
基金
浙江省质量监督检验系统科研计划项目(项目编号:20080228)
关键词
超高效液相色谱-串联质谱
δ-9-四氢大麻酚
大麻酚
大麻二酚
食用油
ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)
δ-9-tetrahydrocannabinol
cannabinol
cannabidiol
edible oil