圩猪及其二元杂交品种猪氟烷基因的基因频率分析

Analysis on Halothane Gene Frequency in Wei Pigs and Its Crossbred Pigs

试验采用PCR-RFLP分子标记技术检测了圩猪(62头)、长白(64头)、杜洛克(61头)、英系大约克(60头)以及长圩零世代(65头)、一世代(121头)、二世代(243头)共676头猪的Hal基因基因型的分布情况。结果表明,在检测的各猪种中,Hal基因显性纯合子频率分别为:1.00,0.71,0.80,0.62,0.91,0.88,0.82;杂合子频率分别为:0,0.29,0.20,0.37,0.09,0.12,0.18。由此说明,虽然氟烷敏感基因频率和基因型频率在长圩3个世代猪种中分布均较低,但Haln基因频率和HalNHaln基因型频率在长圩3个世代中仍呈上升趋势,应进一步采用标记辅助选择方法降低Haln基因频率,直至完全剔除Haln基因。

A total of 676 pigs including 62 Wei pigs, 64 Landrace pigs, 61 Duroc pigs, 60 British Large Yorkshire pigs, 65 Changwei pigs of the zero generation, 121 Changwei pigs of the first generation; and 243 Changwei pigs of the second generation were selected. Distribution of Hal gene genotypes was detected by PCR-RFLP. In the detected swine breeds, the frequency of dominate homozygote of Hal gene was 1.00, 0.71, 0.80, 0.62, 0.91, 0.88 and 0.82, respectively. The frequency of heterozygote was 0, 0.29, 0.20, 0.37, 0.09, 0.12 and 0.18, respectively. Although the allele frequency and genotype frequency of halothane sensitive gene were low in the Changwei pigs of the three generations, the allele frequency of Hal and the genotype frequency of HalN Haln showed a rising trend in the three generations. As a result, the marker-assisted selection should be adopted to reduce the gene frequency of Haln until it is eliminated entirely.