5种硫酸化多糖刺激鸡外周血淋巴细胞增殖的效果比较

The effective comparison of five sulfated polysaccharides to stimulate chicken peripheral lymphocyte proliferation

比较了硫酸化黄芪多糖(sAPS)、硫酸化淫羊藿多糖(sEPS)、硫酸化香菇多糖(sLNT)、硫酸化当归多糖(sCAPS)和硫酸化枸杞多糖(sLBS)体外对鸡外周血淋巴细胞增殖的影响。首先测定5种硫酸化多糖对淋巴细胞的安全浓度,然后取安全浓度范围内的各硫酸化多糖分别单独或与ConA一起加入到淋巴细胞的培养体系中,用MTT法测定淋巴细胞增殖的变化。结果表明,5种硫酸化多糖在合适的剂量均可单独或协同ConA刺激淋巴细胞增殖,且有一定的量效关系,以sAPS60效果最好,sEPS5次之。

In order to select the sulfated polysaccharide（sPS） with the best immunoenhancement,the effects of sulfated astragalus polysaccharides（sAPS）,sulfated epimedium polysaccharide（sEPS）,sulfated lentinan（sLNT）,sulfated Chinese angelica polysaccharide（sCAPS） and sulfated Lycium barbarum polysaccharide（sLBPS） on checken peripheral lymphocyte proliferation were compared in vitro.After the safe concentrations were tested,five sPSs within safe concentration scope were added into the cultivation system of chicken peripheral lymphocyte singly or synergistically with ConA.The lymphocyte proliferations were determined by MTT method.The results showed that five sPSs at proper dose could stimulate the lymphocyte proliferation singly or synergistically with ConA,and there was definite dose-effect relationship.sAPS60 had the best effect,and then sEPS5.