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BDNF和NGF对牛颗粒细胞体外生长的影响 被引量：4

Effects of NGF and BDNF on proliferation of bovine granulosa cells
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摘要 在DMEM培养液中分别添加不同质量浓度的脑源性神经生长因子(Brain-derived neurotrophic factor,BD-NF)和神经生长因子(Nerve growth factor,NGF),观察其对牛颗粒细胞体外生长的影响。结果显示:在DMEM中添加20μg/L的BDNF,可促进牛颗粒细胞的体外生长;在DMEM中添加5μg/L的NGF,可促进牛颗粒细胞的体外生长。结果表明,在DMEM培养液中添加一定质量浓度的NGF和BDNF可促进牛颗粒细胞的体外生长。 Effects of BDNF and NGF concentrations on proliferation of bovine granulosa cells were studied.Supplementation of DMEM cultured media with BDNF at the concentration of 20 μg/L or NGF at the concentration of 5 μg/L caused a significant increase in number of granulosa cells after cultrued for 48 h,indicating that the NGF and BDNF can promote in vitro proliferation of bovine granulosa cells.
作者 易康乐 陈华海 李纯锦 孙艳玲 陈露 石德顺 周虚
机构地区 吉林大学畜牧兽医学院 湖南省畜牧兽医研究所 广西大学动物繁殖研究所
出处 《中国兽医学报》 CAS CSCD 北大核心 2010年第11期1544-1546,共3页 Chinese Journal of Veterinary Science
基金 国家"863"计划资助项目(2008AA101003) 国家科技支撑计划资助项目(2008BADB2B09-08)
关键词 BDNF NGF 牛 颗粒细胞 BDNF NGF bovine granulosa cells
分类号 S814.8 [农业科学—畜牧学]
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参考文献13

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二级参考文献39

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共引文献14

同被引文献54

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引证文献4

二级引证文献8

中国兽医学报
2010年 第11期

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