不同时间经皮穴位电刺激抗大鼠慢性运动性疲劳以及对海马和下丘脑形态与5-羟色胺表达的影响

Different-time Transcutaneous Electrical Acupoint Stimulation for Chronic Sports Fatigue and its Effect on the Morphology and 5-Hydroxytryptamine Content of Hippocampus and Hypothalamus in Rats

目的观察不同时间经皮穴位电刺激(TEAS)抗大鼠运动性疲劳作用以及对海马和下丘脑形态和5-羟色胺(5-HT)含量的影响。方法采用7周递增负荷跑台法建立大鼠慢性运动性疲劳模型,第6周开始,分别在运动后即刻和3 h后TEAS治疗,治疗方法采用经皮神经电刺激仪刺激大鼠足三里穴,治疗持续2周。HE染色观察大鼠海马、下丘脑组织形态学变化,免疫组化法观察海马、下丘脑5-HT的表达。结果 TEAS可以明显减轻运动引起的大鼠海马、下丘脑组织形态损伤,降低大鼠海马、下丘脑5-HT表达,运动后即刻和3 h后治疗的两治疗组间无显著差异。结论 TEAS有促进疲劳恢复提高运动能力的作用。TEAS改善大鼠的运动性疲劳状态与降低中枢5-HT表达、减少神经细胞损伤有关。

Objective To investigate the effect of different-time transcutaneous electrical acupoint stimulation （TEAS） against chronic sports fatigue and on the morphology and 5-hydroxytryptamine （5-HT） content of hippocampus and hypothalamus in rats. Methods A rat model of chronic sports fatigue was made by 8-week load increment platform exercises. From the sixth week, TEAS was given immediately and 3 hrs after the exercise. Rat point Zusanli was stimulated using a transcutaneous electrical nerve stimulator. The treatment lasted 2 weeks. Rat hippocampal and hypothalamic morphology and 5-HT expressions were examined by HE staining and immunohistochemical method, respectively. Results TEAS could significantly reduce the morphological injury to and the 5-HT expressions of rat hippocampus and hypothalamus. There was no statistically significant difference between the two groups treated immediately and 3 hrs after the exercise respectively. Conclusions TEAS functions to promote the recovery from fatigue and improve the motor ability. TEAS reducing rat chronic sports fatigue is related to its decreasing central 5-HT expressions and neurocyte injuries.