摘要
以洞庭湖和原种场长吻(Leiocassis longirostris)为研究对象,采用20个随机引物对10个野生个体进行了随机扩增多态性DNA(random amplified polymorphic DNA,RAPD)群体遗传多样性分析。共检测到103条带,每个引物产生的条带数在2～9之间,片段大小在0.2～3.0kb之间,多态座位比例为41.75%,2群体内个体间遗传相似系数和遗传距离分别是,洞庭湖个体间遗传相似性系数(S)0.8795～0.9833,遗传距离(D)0.0167～0.1205;原种场个体间S为0.8794～0.9892,D为0.0319～0.1108;比较2群体S为0.7983～0.9994,D为0.0167～0.3017;Nei遗传多样性指数(He)0.3269。结果表明,长吻群体内遗传多样性较为丰富。
Taking wild Leiocassis longirostris from Dongting Lake and original breeding farm as samples and with 20 random primers,we conducted a RAPD analysis of genetic diversity of 10 wild individuals,finding 103 bands whose fragment size are 0.2～3.0 kb,and that each primer generates 2～9 bands with a polymorphic loci proportion of 41.75%.Genetic similarity coefficient and genetic distance within 2 populations are as follows：genetic similarity index（S） of Dongting Lake individuals varies from 0.879 5 to 0.983 3 and that of original breeding farm ones varies from 0.879 4 to 0.989 2;genetic distance（D） varies from 0.016 7 to 0.120 5 and that of original breeding farm ones varies from 0.031 9 to 0.110 8;the genetic similarity coefficient of 2 populations is 0.798 3～0.999 4 and the genetic distance is 0.016 7～0.301 7;Nei genetic diversity index is 0.326 9.The results indicate that L.longirostris has relatively high genetic diversity within populations.
出处
《南方水产》
2010年第6期77-80,共4页
South China Fisheries Science
基金
湘南学院院级一般项目(2007Y031)
