SD大鼠原代颌下腺细胞体外培养方法的改良

Improving SD RAT Submandibular Gland Cells in Primary Culture Method

目的研究SD大鼠颌下腺细胞体外培养的方法，使细胞能顺利的在体外培养并能提供有功能的神手细胞。方法获取新生SD大鼠幼仔颔下腺组织，经酶消化后培养，以PBS和DMEM＋F12为冲洗液，比较两者对细胞生长的影响。结果体外培养的颌下腺细胞以DMEM＋F12为冲洗液细胞能持续的生长。结论体外培养的颔下腺细胞以DMEM＋F12为冲洗液能获得增殖活跃传代细胞，并为颌下腺研究提供种子细胞。

Obeetive To study the SD rat submandibular gland cells in vitro method, the cells can be successfully cultured in vitro and provide the functional seed cells. Methods Neonatal SD rat pups submandibular gland tissue by enzymatic digestion and cultured, with PBS and DMEM ＋ F12 as the washing liquid, to compare the effects on cell growth. Results The cultured submandibular gland cells to DMEM ＋ F12 for the fluid cell can continue to grow. Conclusion In vitro cultured submandibular gland ceils DMEM ＋ F12 to get active proliferation fluid passage cells, can provide seed for the submandibular gland cells.