摘要
以四季蜜龙眼花芽为材料,进行了总RNA的提取与纯化,应用RT-PCR技术扩增其3′端序列,经测序和拼接,获得SAM3′末端,共1 253 bp;序列分析表明,与杨树SAM基因同源性达到96%,为龙眼成花机理的深入研究奠定了基础。
The extraction and purification of the RNA of flower bud of Dimocarpus longan Lour. cv Sijimi were conducted. Utilizing RT-PCR technique to expand 3＇ end sequence of SA M,testing its sequence and putting them together,the 3＇end of SAM at the amount of 1 253 bp was attained. The nucleotide sequence comparison with the SAM gene of Populus trichocarpa showed that identity was higher than 96% ,which would provide the basis to further study on the mechanism of blossom formation.
出处
《现代农业科技》
2010年第23期118-120,共3页
Modern Agricultural Science and Technology
基金
福建省科技重点项目"四季蜜龙眼成花机理与配套栽培技术研究"(基金编号:2008N01041075)
关键词
四季蜜龙眼
成花相关基因
SAM基因
Dimocarpus longan Lour. cv Sijimi
the relevant gene to floral bud formation
SAM gene
