银杏悬浮细胞系的建立及其黄酮糖苷的产生

Building of Suspension Cell Line in Ginkgo biloba and Producing of the Flavonol Glycosides

在固体培养基中添加５ｍｌ·ｌ-1原汁的蜂蜜，可加快细胞的生长速度，获得黄绿色、疏松、旺盛生长的愈伤组织，四种浓度的Ｎａ２Ｓ２Ｏ３·５Ｈ２Ｏ处理与对照的倍增时间呈非常显著差异。通过细胞悬浮培养比较，在Ｓ２中细胞倍增时间为２．４６ｄ，生长最快，细胞在Ｓ２、Ｓ４、Ｓ７、Ｓ８、Ｓ９、Ｓ１０、Ｓ１１、Ｓ１２培养基中能连续正常继代生长，但生长速度有差异，在继代１５次时，合并收集悬浮细胞，经气相色谱分析，银杏黄酮糖苷含量达到０．７０％，接近叶片中的含量水平（０．７５％）。

Experiment proved that when the additament of 5 ml. l -1 honey was added to the agarmedia, the population cell doubling time was clearly less than that of the control and the yellowish,loose and active callus was formed. There were very singnificant differences (P>0. 01) between the treatments of Sodium Thiosalfate and control in doubling time. The doubling time in theS2 medium was 2. 46 d, the suspension cells could be subcultured normally in S2.S4.S7.S8.S9.S10.S11 and S12 media, after 15 times, the suspension cells were harvested, the aglycones wereanalysed by gas chromatograph, the results showed that the content of the flavonol glycosides was0. 70 %, which approached the level of intact plant leaves (0. 75 %).