木香中总氨基酸的含量测定

Determination of Total Amino Acid in Aucklandia lappa Decne

目的：建立内蒙古木香中总氨基酸的含量测定方法。方法：采用紫外可见分光光度法测定，检测波长为563nm。结果：L-谷氨酸在浓度6．160—18．480μg／ml范围内呈良好线性关系。回归方程Y=35．909x-0．1784（r=0．9992）。线性关系良好，平均加样回收率为98．79％，RSD为1．74％（n=6）。该法测得木香中总氨基酸含量为1．73％，与氨基酸自动分析仪所测得的木香中总氨基酸的含量一致。结论：该方法灵敏、简便、快速、准确可靠。

Objective：To establish a method for determination of total amino acid in Aucklandia appa Decne.Method：using ultraviolet and visible spectrophotometric determination, detecting at wavelength of 563nm.Result： L-glutamate had a liner relationship with the absorbance in the range of 6.160-18.480？ g/ml and the regression equation was as follows：Y=35.909x-0.1784 （r=0.9992）.The average recovery for meletin was 98.79%, RSD= 1.74%.Canclusion：The method is simple, sensitive, accurate and can be used for total flavones determination in Aucklandia lappa Decne.