单抗阻断ELISA检测副鸡嗜血杆菌血清型特异性抗体的研究

Monoclonal Antibodies Blocking ELISA for the Detection of Serovar specific Antibodies to Haemophilus paragallinarum

本研究在副鸡嗜血杆菌Ａ、Ｃ型特异性单克隆抗体的基础上成功地建立了能区分Ａ、Ｃ血清型抗体的阻断ＥＬＩＳＡ诊断方法，并应用此法对９种常见病原体阳性血清及多份免疫鸡、人工感染鸡、临床可疑病鸡血清和阴性血清进行了检测，结果证明，本方法具有较好的特异性和敏感性，而且操作简便、快速，是一种适用于鸡传染性鼻炎的诊断、流行病学调查、检疫和监测的良好方法。

A serovar specific blocking enzyme linked Immunosorbent assay(B ELISA) based on two monoclonal antibodies was developed in this study to detect antibodies to serovar A or C of Haemophilus paragallinarum ,positive serums to 9 usual pathogens and many serum samples derived from vaccinated and artificially infected chickens,immunized and sick clinical chickens were tested by it.The results showed that this B ELISA,which with good specificity,sensitivity and simplicity,would be a useful assay for diagnosis,monitoring and epidemiologic analysis of infectious coryza.