迟缓爱德华氏菌检验程序的研究

Studies on the Procedures for the Detection of Edwardsiella tarda

本试验研究了迟缓德华氏菌（Edwardsiellatrada，Et）的细菌学及致病因子检测，确定了相应检测指标，制定了检验程序。对不同来源的Et作分离及生化鉴定，确定10项生化鉴定指标。用三种方法即平板法（PlateAssay，PA）、接触法（ContactHemolysis，CH）和上清法（SunernatantAs－say，SA）检测Et溶血素，阳性率依次为75％、75％、57．14％。28株Et中，18／26的胞外产物（Extra－cellularProducts，ECP）对HEP－2细胞有细胞毒性，17／28菌株有侵袭力。用ATCC15947株ECP的抗血清进行Det－ELISA，检测阳性率为19／26（73．08％）。

Procedures for the deflection of Edwardsiella tarda (Et) including routine bacterial detection and virulent factors detection were established and evaluated for 28 strains. Et strains must be Gram's negative, with motility. And 10 biochemistry tests to idntify Et were determined based on the isolation and identification. Plate assay (PA ), contact hemolysis(CH)and supernatant assay (SA ) were used for detecting haemolysin,and the detection rates were 75 %, 75 % and 57. 14 % respectively of tested strains 17/28 could invade HEp-2 cells,and the ECP of 18/26 was cytotoxic. The positive rate of Dot-ELISA detecting ECP of Et with antiserum against the ECP of ATCC15947 was 73. 08%.