江西地方猪种(群)的RAPD分析及其品种分类研究

RAPD Markers for the Study on Genetic Relationships among Some Native Pig Populations in Jiangxi Province

用RAPD技术分析了江西地方8个猪种(群)的基因组池DNA的多态性。遗传距离指数计算和聚类分析结果表明,长白猪和新太湖猪的遗传分化明显,与其他猪群相距较远;而上高两头乌与新余两头乌、乐平和德兴花猪与修水杭猪亲缘关系较近。综合现行分类方法以及采用生化和免疫遗传标记分析的结果,认为上高两头乌与新余两头乌、乐平花猪和德兴花猪可分别归并为同一品种,不支持东乡花猪归并为赣东北花猪类型。

Random amplified polymorphic DNAs (RAPDs) assay was used to detect bulked genomic DNA polymorphism of eight pig breeds (or populations) including Leping Spotted, Dexing Spotted, Dongxiang Spotted, Hang, Shanggao Black head - hip, Xinyu Black head - hip, New Taihu and Landrace. 16 primers generated 44 polymorphic markers from screening of 80 primers. The genetic distance index matrix and UPMGA dendrogram indicated that the genetic differentiation between Landrace, New Taihu and the other native pig populations examined was remarkable, while Shanggao Black head - hip and Xinyu Black head - hip population, Leping Spotted, Dexirig Spotted and Hang had closer genetic relationships respectively. With the consideration of previous results based on biochemical and immunogenetical genetic markers, It could be inferred that Shanggao Black head - hip and Xinyu Black head - hip pig population could be classified into one breed, as the same to Leping Spotted and Dexing Spotted population. However, Dongxiang Spotted and Leping Spotted should not be classified into the same breed, the genetic character of Dongxiang Spotted should be further evaluated.