共培养血管内皮细胞和胰岛对大鼠胰岛早期功能的影响

Improve islet survival and function with rat endothelial cells in vitro coculture

目的探讨通过体外共培养胰岛和血管内皮细胞能否改善胰岛的功能。方法将远交群（SD）大鼠实验分为两组：A组为单纯胰岛组;B组为胰岛和内皮细胞共培养组。首先从分离培养大鼠的血管内皮细胞,然后和分离纯化后胰岛进行培养,通过吖啶橙/碘丙啶（AO/PI）染色和胰岛素释放实验来判断胰岛的活性。结果单纯培养组胰岛和共培养组胰岛在1周内显示了良好的形态。培养14d,共培养组胰岛形态依然完整,折光性好;但单纯胰岛培养组胰岛形态不规则,甚至松散、解体。90﹪的胰岛通过AO/PI染色显示良好的活性;胰岛素释放实验显示：除第1天外共培养组和单纯培养组刺激指数有显著性的差异（P〈0.05）。结论血管内皮细胞和胰岛共培养后能大大的改善胰岛的存活和功能。

Objective To investigate whether the function of isolated islets can be retained by co-culture with thoracic aorta endothelial cells （AEC） in vitro. Methods SD rats were used in this study. The islets were standard culture （group A） or co-cultured with ECs （group B）.The endothelial cells （ECs） were isolated from thoracic aorta. Islets were purified and cultured with ECs, Then the viability of the islets was assessed by AO/PI double staining and by insulin release assay. Results Islets in group B exhibited normal morphology, with greater than 90﹪ staining positive as detected by AO/PI with 7 days. Insulin release assay showed that： there was a significantly higher simulation index （SI） in group B as compared with group A （P 0.05） except the first day. Conclusion This study suggests that co-cultrue of freshly isolated rat islets with ECs can improve post-culture survival and islet function in vitro.