摘要
通过对从菲律宾Laguna 省水稻植株上分离获得的700 个细菌菌株进行离体与生物测定发现:在水稻生态系统中,存在着大量格兰氏阴性、非致病性、对纹枯病有拮抗作用的细菌。用Biolog 微板的方法鉴定了195 个细菌菌株的种类。在147 个格兰氏阴性菌株中有14 个属39 个种,在48 个格兰氏阳性菌株中有8 个属13 个种。在195 个被测菌株中最为常见的种是Stenotrophomonas maltophilia ,Pseudomonas aeruginosa 和Pseudomonas pudita ,并且上述3 个种内具有拮抗性能较强菌株的比例相当高。因此,这3 个种的细菌对水稻病害的生物防治很有潜力。用PCR 的方法分析上述3 个细菌种部分菌株的生物多样性,结果表明:同一属不同种菌株之间的DNA 带谱有一定的交叉;不同属之间的DNA带谱的差异明显大于种之间的差异。
Seven hundred bacterial strains isolated from leaf and sheath of rice plants of Laguna province, Philippines were tested in vitro.Results showed that there were a great number of bacteria that were Gram negative、nonpathogenic and antagonistic to Rhizoctonia solani. Among 195 bacteria strains identified,147 were Gram negative,belonging to 14 genera 39 species, and 48 were Gram positive belonging to 8 genera 13 species. Stenotrophomonas maltophilia , Pseudomonas aeruginosa and Pseudomonas pudita were the predominant species possessed large amount of antagonistic strains. The biological diversities of partial strains of above species were analyzed by rep-PCR. Results showed that DNA bands of different species from same genus crossed each other at sometime; the differences of DNA bands were greater between genus than that between species.
出处
《植物病理学报》
CAS
CSCD
北大核心
1999年第2期97-103,共7页
Acta Phytopathologica Sinica
关键词
拮抗细菌
生物多样性
水稻纹枯病
生物防治
antagonistic bacteria, population distribution, biological diversity, Rice sheath blight, biological control