摘要
利用CMVFny 株系RNA3 全长cDNA 克隆, 构建了运动蛋白(MP) 基因5′端缺失突变体和3′端缺失突变体的原核表达载体。SDS- PAGE 分析表明, 经IPTG 诱导, MP 基因及其2 种缺失突变体均能在大肠杆菌BL21(DE3) pLysS中高效表达。利用分离包含体的方法, 提纯了全长的及C 端缺失的MP。光密度扫描分析表明, 提纯产物的纯度达96 .6 % 。
Two expression vectors containing 5′ regional deletion and 3′ regional deletion of cucumber mosaic virus movement protein (MP) gene were constructed respectively. With IPTG induction, the full length of MP and its two mutants were overexpressed in Escherichia coli BL21(DE3) pLysS respectively. Using a method to separate the inclusion bodies from the rest of the bacterial proteins by centrifugation, the MP and its C terminal deletion mutant were purified. The purity was about 96.6% estimated by Shimadzu 910 scanning.
出处
《植物病理学报》
CAS
CSCD
北大核心
1999年第2期132-137,共6页
Acta Phytopathologica Sinica
关键词
黄瓜花叶病毒
运动蛋白
突变体
原核表达
基因
cucumber mosaic virus
movement protein
mutants
prokaryotic expression
purification