芩丹胶囊对老年自发性高血压大鼠血管外膜Ⅰ、Ⅲ型胶原表达的影响

Effect of Qindan Capsule on Expression of Vascular Adventitial CollagenⅠand Ⅲ in Old Spontaneous Hypertensive Rats

目的观察芩丹胶囊对老年自发性高血压大鼠(SHR)血管外膜Ⅰ、Ⅲ型胶原蛋白和基因表达的影响,并进一步探讨血管外膜重构的可能作用机制。方法 32只40周龄老年自发性高血压大鼠随机分为模型组、芩丹胶囊大剂量组[750 mg/(kg·d)]、芩丹胶囊小剂量组[150 mg/(kg·d)]及氯沙坦组[30 mg/(kg·d)],每组8只;另设老年Wistar-Kyoto大鼠为空白对照组和正常药物对照组[芩丹胶囊750 mg/(kg·d)];模型组和空白对照组给予等量生理盐水灌胃,每天1次,共12周。观测各组大鼠收缩压;免疫组化法观察Ⅰ、Ⅲ型胶原在胸主动脉外膜的蛋白表达以及PCR检测mRNA表达。结果与模型组比较,芩丹大、小剂量组及氯沙坦组收缩压均明显下降(P<0.05);与模型组比较,此3组免疫组化中Ⅰ、Ⅲ型胶原蛋白表达显著降低(P<0.05),PCR显示Ⅰ、Ⅲ型胶原mRNA在外膜表达也有所降低(P<0.05)。结论芩丹胶囊不仅能够有效地降低SHR大鼠血压,而且从Ⅰ、Ⅲ型胶原表达量明显减少可看出芩丹胶囊对动脉血管外膜重构有明显的抑制及逆转作用。

Objective To investigate the effect of Qindan Capsule（QDC） on gene and protein expression of vascular adventitial collagenⅠand Ⅲ（VAC1 and VAC3） in spontaneously hypertensive rats（SHR）,for further research the possible mechanism of vascular adventitial fibroblasts remodeling.Methods Thirty-two SHR,40 weeks old,were equally randomized into the model group and the three treated groups treated respectively with high（750 mg/Kg·d） and low dosage QDC（150 mg/Kg·d）,and losartan（30 mg/Kg·d）,once a day for 12 weeks.Besides,a normal blank control group and a normal QDC（750 mg/Kg·d） medicated group were set up with same aged Wistar-Kyoto rats.Systolic blood pressures of rats were monitored,gene and protein expressions of VAC1 and VAC3 in rats’ thoracic aortic adventitia were detected at the end of experiment using immune-histochemical staining and real-time quantitative fluorescent PCR respectively.Results Compared with the model group,blood pressure as well as the gene and protein expressions of VAC1 and VAC3 were all lower in the two QDC（high and low dosage） treated groups（P0.05）.Conclusion QDC could not only effectively reduce the blood pressure in SHR,but also suppress and even reverse their thoracic aorta adventitial vascular remodeling,which is displayed by the obvious lowering of VAC1 and VAC2 expression levels.