华蟾素对子宫内膜癌Ishikawa细胞株裸鼠皮下移植瘤生长及RRM2基因表达的影响

Effect of Cinobufotalin on Growth of Xenograft of Endometrial Carcinoma Cell Line Ishikawa in Nude Mouse and Its Impact on RRM2 Expression

目的研究华蟾素对子宫内膜癌Ishikawa细胞株裸鼠皮下移植瘤生长的抑制作用及对肿瘤组织中核糖核苷酸还原酶亚单位M2(ribonucleotide reductase subunit M2,RRM2)基因表达的影响。方法应用人子宫内膜癌Ishikawa细胞株建立子宫内膜癌裸鼠皮下移植瘤模型,随机将11只裸鼠分为2组,分别应用华蟾素和生理盐水瘤体内注射治疗1周。观察治疗前后移植瘤体积的变化;计算肿瘤增殖抑制率;RT-PCR方法检测肿瘤组织中RRM2 mRNA表达;Western blot方法检测RRM2蛋白表达。结果华蟾素治疗组移植瘤体积为(0.1314±0.0304)cm3,对照组为(0.3600±0.1145)cm3,两组比较差异有统计学意义(P<0.05);治疗组肿瘤增殖抑制率为43.46%。治疗组肿瘤组织中RRM2 mRNA及蛋白质光密度比值与对照组比较差异均有统计学意义(P=0.019,P=0.001)。结论华蟾素具有抑制子宫内膜癌裸鼠皮下移植瘤生长的作用,这可能与其抑制肿瘤组织中RRM2表达有关。

Objective To investigate the inhibitory effect of cinobufotalin（CBT） on the growth of xenograft endometrial carcinoma cell line ishikawa in nude mice,and its impact on the expression of ribonucleotide reductase subunit M2（RRM2）.Methods Eleven nude mice with xenograft were randomly divided into two groups,the CBT group and the control group,which received intra-tumor injection of CBT and saline respectively for one week.The sizes of xenografts were measured before and after the treatment to calculate the inhibition ratio of tumor proliferation;the RRM2-mRNA and protein expressions in tumor tissue were measured by RT-PCR and Western blot respectively.Results After treatment,the size of xenografts in the CBT group was（0.1314±0.0304） cm3,which was significantly lower than that in the control group（0.360 0±0.1145） cm3,（P0.05）,the tumor proliferation inhibition ratio being 43.46%.The differences of RRM2 mRNA and protein expression levels between the two group were significant（P=0.019 and P=0.001）.Conclusion CBT significantly inhibits the growth of the xenografts of endometrial carcinoma Ishikawa in nude mice,and the action mechanism is possibly associated with the inhibition on RRM2 expression.