CD4^＋T细胞因子在胆道闭锁患儿肝脏组织中的表达及其意义

Analysis of cytokines produced by CD4^＋ T cells in liver tissues of the patients with biliary atresia

目的 检测T淋巴细胞中的11种CD4^＋T细胞因子在胆道闭锁（biliary atresia,BA）患儿肝脏组织中的表达,以探讨其在胆道闭锁发病机制中的意义.方法 在病理的基础上,采用流式微球技术对29例BA及9例对照组患儿肝脏组织CD4＋T细胞表达的11种细胞因子（IL-12p70、IFN-γ、IL-2、IL-10、IL-8、IL-6、IL-4、IL-5、IL-1β、TNF-α和TNF-β）同时进行定量检测,并对其代表因子IFN-γ进行免疫组化定位分析.结果 CD4^＋T细胞表达的11种细胞因子中,BA组患儿肝脏组织中IL-1β、IL-2、IL-6、IL-8、IFN-γ、TNF-α以及Th1细胞因子总量（IL-1β、IL-2、IL-8、IL-12p70、IFN-γ、TNF-α、TNF-β）与促炎因子总量（IL-1β、IL-2、IL-6、IL-8、IL-12p70、IFN-γ、TNF-α、TNF-β）分别为2920.69、1 106.01、152.22、12 614.22、834.18、161.29、19 504.55、19653.06,数值明显高于对照组的1 096.00、243.68、5.98、965.17、147.28、30.56、2 617.93、2 623.91,差异具有统计学意义（P＜0.05）.结论 由CD4＋Th1细胞及其细胞因子所介导的针对胆道上皮细胞的免疫炎症性疾病可能是造成胆道闭锁的主要原因之一.

Objective To investigate the cytokines produced by CD4 ＋ T cells in liver tissues of the patients with biliary atresia （BA）. Methods The liver tissues of the 29 patients with BA were grounded and centrifuged. Then, the supernatants were collected. Nine patients not affected BA served as controls. The 11 cytokines produced by CD4 ＋ T cells including IL- 12p70, IFN-γ, IL-2, IL- 10, IL-8,IL-6, IL-4, IL- 5, IL-1 β, TNF-α and TNF-β were detected using microsphere-based flow cytometry. Results Of the BA patients, the amount of IL-1β, IL-2, IL-6, IL-8, IFN-γ and TNF-α were 2 920. 69,1 106. 01,152. 22,12 614. 22,834. 18 and 161.29, respectively. The total amount of the Th1 cytokines including IL-1β, IL-2, IL-8, IL-12p70, IFN-γ, TNF-α and TNF-β was 19 504. The total amount of the proinflammatory cytokines including IL-1β, IL-2, IL-6, IL-8, IL-12p70, IFN-γ, TNFα and TNF-β was 19 653. 06. The amounts of these cytokines were significantly higher than those of the control group,which were 1 096.00,243.68,5.98,965.17,147. 28 ,30. 56,2617. 93, and 2 623. 91, respectively. Conclusions The increased expressions of the cytokines expressed by the CD4 ＋ Thl cells may be one of the main causes of BA.