机采血小板实验室保存期间的生化改变

The Biochemistry Changes of Apheresis Platelets within the Storage Time

目的探讨机采血小板在实验室内保存期间的生化改变及其意义。方法采用ROCHE cobas b221和OLYMPUS AU400测定血小板样本的pH值、PO2、PCO2、葡萄糖(GLU)浓度、乳酸脱氢酶(LDH)释放量。结果保存期间pH值由7.311(第1天)降至7.116(第5天);PO2波动于106.7～119.5mmHg(1mmHg=0.133kPa);PCO2波动于25.0～35.8mmHg;GLU浓度呈进行性下降,由18.8mmol/L降至15.2mmol/L;LDH浓度显著增加,由150mmol/L(第1天)增至259mmol/L(第5天)。结论血小板在保存期内,其生化参数会随着保存时间发生改变。而这些参数作为间接指标对机采血小板是否被细菌污染具有一定的指导意义。

Objective To observe the biochemistry changes of apheresis platelets（AP）,including pH、PO2、 PCO2、GLU、LDH,within the storage time and its significance.Methods The pH、PO2、PCO2、GLU and LDH of platelet samples were detected by ROCHE cobas b 221and OLYMPUS AU400.Results During the storage time,the pH value of platelet decreased gradually from 7.311（the first day）to 7.116（the fifth day）;the PO2fluctuated from 106.7mm Hg（1mm Hg=0.133kPa）to 119.5mm Hg;the PCO2fluctuated from 25.0mm Hg to 35.8mm Hg;the concentration of GLU decreased from 18.8mmol/L to 15.2mmol/L;the concentration of LDH increased observably from 150mmol/L（the first day）to 259mmol/L（the fifth day）.Conclusion There were observably changes of biochemistry of the AP during storage time,and these changes may be the indicators of platelets contamination.