仪器法计数幼稚粒细胞对白血病患者外周静脉血的适用性探讨

Comparison between Instrument Method and Microscope Test Method in Measuring the Percentage of Immature Granulocytes of Peripheral Venous Blood in Patients with Leucocythemia

目的探讨仪器法和镜检法计数白血病幼稚粒细胞百分比的相关性和一致性。方法 2009年69月对71例慢性粒细胞性白血病(慢粒)、亚急性粒细胞性白血病(M2)、急性早幼粒细胞性白血病(M3)及急性粒单核细胞性白血病(M4)白血病患者进行仪器法和镜检法计数周围静脉血幼稚粒细胞的百分比的检测,并进行比较分析。结果两种方法计数的慢粒、M2、M3及M4型共71例白血病患者的幼稚粒细胞的百分比比较,有统计学意义(t=6.404,P<0.01);但具有相关性(r=0.771,P<0.001)。且四种类型白血病中的每一种类型的白血病的两种方法的幼粒值也都具有相关性和不一致性(P<0.05)。结论 SYSMEX XE-2100全自动血细胞分析仪对慢粒、M2、M3及M4的周围静脉血幼稚粒细胞识别能力欠佳,仍需采用镜检法进行检测。

Objective To investigate the correlation and consistency of the percentage of immature granulocytes of peripheral venous blood in patients with leucocythemia between the instrument method and microscope test method. Methods From June to September 2009,instrument method and microscope test method were used to measure the percentage of immature granulocytes of peripheral venous blood in patients with leucocythemia[chronic granulocytic leukemia（CGL）,subacute granulocytic leukemia（M2）,acute promyelocyte leukemia（M3）,and acute myelomonocytic leukemia（M4）].Results The difference in the percentage of immature granulocytes of the 71samples between the 2methods was significant（t=6.404,P0.01）,but still with correlation（r=0.771,P0.001）.Besides,there were also correlation and consistency of the percentage of immature granulocytes of the four types of leukemia between the two methods（P0.05）.Conclusion The identification ability of SYSMEX XE-2100type automatic blood cell analyzer for measuring the percentage of immature granulocytes of peripheral venous blood in patients with CGL,M2,M3and M4is still weak.Currently,microscope test method still needs to be applied in the measurement.