中国马褂木精细胞分离技术

Mass Isolation of Viable Sperm Cells from the Pollen of Liriodendron chinense

自８０年代以来，被子植物的精细胞分离和生物学研究已成为植物受精工程中重要的研究内容。在以前的基础上，为获得大量的中国马褂木精细胞，本研究通过蔗糖密度梯度离心法纯化萌发花粉管，用研磨法获取大量有生活力的精细胞，并利用梯度离心法纯化生活精细胞。利用这些方法所获得的精细胞在室温的环境下可以存活４ｈ左右。

Since 80's,the isolation and biological studies of sperm cells in angiosperms have been an important research area in plant fertilization engineering. Based on the isolation of vialbe sperm of Liriodendron chinense, the mass isolation of viable sperm cells form the its pollen was reported. With sugar density gradient centrifugation method, the germinated pollen was firstly purified. And the vialbe sperm were released by a means of the grinding method. The viable sperm was purified with sugar density gradient centrifugation. The testing result shows that the isolated sperm cells might survive for 4h at room temperature.