盐水胁迫下接种AM真菌对牡丹幼苗抗氧化酶活性的影响

Effects of Arbuscular Mycorrhizal Fungi on Antioxidant Enzyme Activity in Peony Seedlings under Salt Stress

将牡丹(Paeonia suffruticosa)幼苗接种丛枝菌根(arbuscular mycorrhizas,AM)真菌摩西球囊霉(Glomus mosseae)和地表球囊霉(G.versiforme)后,在4个不同盐水处理(质量百分比0、8%、16%和24%)下,研究AM真菌对牡丹抗氧化酶活性的影响。研究结果表明,8%盐水处理,牡丹幼苗菌根依赖性最高,且接种G.mosseae的处理显著高于接种G.versiforme的处理,分别为172%和150%;该胁迫30d时,接种G.mosseae和G.versiforme植株干质量分别为0.51和0.45g,叶片相对含水量分别为80.5%和78.5%,叶片超氧化物歧化酶(SOD)的活性分别为4.72和4.46U·g-1,过氧化物酶(POD)活性分别为60.3和57.4U·min-1·g-1,过氧化氢酶(CAT)活性分别为51.3和47.2U·min-1·g-1,均显著高于对照。16%和24%盐水处理下的表现与此相似。随盐胁迫时间的延长,SOD和CAT活性呈先升高后降低趋势,POD活性呈持续上升趋势。AM真菌通过增强牡丹幼苗抗氧化酶活性,提高其耐盐性,以G.mosseae接种效果较好。

The effects of arbuscular mycorrhizal（AM） fungi on antioxidant enzyme activity in peony（Paeonia suffruticosa） seedlings inoculated with Glomus mosseae or G.versiforme,and grown under the condition of four levels of artificial seawate（r0,8%,16% and 24%） were studied.The experimental results showed that mycorrhizal dependence（MD） of peony seedlings in treatment with 8% salt was the highest,and MD of peony seedlings inoculated with G.mosseae was 172% significantly higher than that with G.versiforme（150%）.Thirty days after salt stress with 8% salt concentration,the dry weight,leaf relative water content,leaf superoxide dismutase activity,peroxidase activity,peroxidase activity,catalase activity of peony seedlings inoculated with G.mosseae and G.versiforme were 0.51 g and 0.45 g,80.5% and 78.5%,4.72 and 4.46 U · g-1,60.3 and 57.4 U · min-1 · g-1,51.3 and 47.2 U · min-1 · g-1,respectively,and significantly higher than control.The similar trends were given under 16% and 24% salt concentration.As time prolonged under salt stress condition,SOD and CAT activities increased first but decreased afterwards,while POD activities showed an increasing trend.AM fungi could increase activities of the antioxidant enzyme system.Thus,the salt tolerance of mycorrhizal seedlings was enhanced.Glomus mosseae was the superior.