20周有氧运动对雌性大鼠心肌组织衰老相关候选基因mRNA表达的影响

Effects of 20 Weeks Aerobic Exercise on the Expression of Candidate Genes mRNA in Cardiac Muscle of Aging Female Rats

目的:探讨20周有氧运动对雌性中老年大鼠心肌组织衰老相关候选基因mRNA表达的影响。方法:健康雌性SD大鼠30只,随机分为3组:青年对照组、中老年对照组、中老年运动组。采用20周递增负荷跑台运动,建立中老年大鼠有氧运动模型。采用实时荧光定量PCR技术,检测心肌组织Ceacam1、Gpbar1、Cebpg、Cdk5r1、Ggt1、Sftpd、Tas2r134、Mc4r、LOC299282、Pawr mRNA含量。结果:与年轻雌性大鼠相比,中老年不运动大鼠心肌组织Cdk5r1、LOC299282、Ceacam1、Pawr mRNA表达显著增加(P<0.01),Sftpd、Mc4rmRNA显著增加(P<0.05);中老年运动大鼠心肌组织LOC299282、Ceacam1、Pawr mRNA表达显著增加(P<0.01),Cdk5r1、Tas2r134、Sftpd、Ggt1 mRNA表达显著减少(P<0.05)。与中老年不运动大鼠相比,中老年运动大鼠心肌组织Cdk5r1、Tas2r134、Sftpd、Ceacam1、Pawr、Mc4r、Ggt1 mRNA表达显著减少(P<0.01),Gpbar1mRNA表达显著减少(P<0.05)。而Cebpg基因表达的改变未见统计学意义。结论:衰老及长期规律的有氧运动可改变Ceacam1、Gpbar1、Cdk5r1、Ggt1、Sftpd、Tas2r134、Mc4r、LOC299282、Pawr mRNA表达,衰老可增加这些基因的表达,规律的有氧运动使增加的基因mRNA表达减少,提示有氧运动可改善心肌组织中衰老相关候选基因因衰老而发生的表达改变。

Objective To study the effects of 20 weeks aerobic exercise on the expression of candidate genes in cardiac muscle of aging female rats.Methods Thirty female SD rats were randomly divided into three groups：young female rats（FYN,3 months old）,female non-exercise rats（FON,14 months old）,and female exercise rats（FOE,14 months old）.Rats in FOE group undertook 20 weeks progressive aerobic treadmill exercise.Real time PCR was applied to exam the expression of Ceacam1,Gpbar1,Cebpg,Cdk5r1,Ggt1,Sftpd,Tas2r134,Mc4r,LOC299282,Pawr mRNA.Results Compared with FYN group,the expression of Cdk5r1,LOC299282,Ceacam1,Pawr mRNA in myocar-dium of FON group increased（P 0.01）,Sftpd,Mc4r mRNA in myocardium of FON increased（P 0.05）.Compared with FYN group,the expression of LOC299282,Ceacam1,Pawr mRNA in myocardium of FOE group increased（P 0.01）,Cdk5r1,Tas2r134,Sftpd,Ggt1 mRNA in myocardium of FOE group decreased（P 0.05）.Compared with FON group,the expression of Cdk5r1,Tas2r134,Sftpd,Ceacam1,Pawr,Mc4r,Ggt1 mRNA in myocardium of FOE group decreased（P 0.01）,Gpbar1 mRNA in myocardium of FOE group decreased（P 0.05）.The expression of Cebpg mRNA in myocardium of all groups kept unchanged.Conclusion Results showed that the pattern of gene expression changed in aged cardiac muscle in response to regular exercise training.