TSLC1和4.1B在非小细胞肺癌中的表达及临床意义

Expressions and Clinical Significances of TSLC1 and 4.1B in Non-small Cell Lung Cancer

背景与目的肺癌肿瘤抑制物1(tumor suppressor in lung cancer-1,TSLC1)属于细胞粘附分子中免疫球蛋白超家族成员,肺腺癌差异表达基因(di erentially expressed in adenocarcinoma of the lung,4.1B)属于NF2/ERM/4.1蛋白超家族成员之一,两者可能通过构建相邻细胞间稳定的粘附作用而抑制恶性肿瘤的发生。本研究通过检测TSLC1和4.1B在非小细胞肺癌中的表达及其与患者临床特征的关系,分析两种基因表达的相关性,以期为临床诊断与治疗提供理论基础。方法采用RT-PCR的方法检测52例非小细胞肺癌组织以及52例相应癌旁正常肺组织中TSLC1和4.1B的表达。结果 TSLC1和4.1B在癌组织中的表达量明显低于癌旁正常肺组织(0.349±0.008vs0.555±0.010;0.209±0.040vs0.721±0.071)(P<0.01)。TSLC1和4.1B的表达与非小细胞肺癌的分化程度、TNM分期有关(P<0.05),而与患者性别、年龄、病理分型无关(P>0.05)。TSLC1与4.1B的表达呈正相关(r=0.471,P<0.001)。结论 TSLC1和4.1B在非小细胞肺癌的发生中起抑制作用,两者可能通过级联反应共同参与了非小细胞肺癌的发生和发展。TSLC1和4.1B有望成为非小细胞肺癌基因诊断与治疗的靶点。

Background and objective Tumor suppressor in lung cancer-1 （TSLC1） belongs to immunoglobulin superfamily of cell adhesion molecule and differentially expressed in adenocarcinoma of the lung （4.1B）belongs to NF2/ ERM/4.1 protein superfamily.They may suppress carcinogenesis via construction of the adjacent cell adhesion stability.The aim of this study is to detect the expressions of TSLC1 and 4.1B in non-small cell lung cancer and the clinical pathological significances.Methods The expressions of TSLC1 and 4.1B were detected by RT-PCR in 52 cases of non-small cell lung cancer and corresponding adjacent cancer lung tissues.Results The expressions of TSLC1 and 4.1B in cancer tissues were significantly lower than that in adjacent cancer lung tissues （0.349±0.008 vs 0.555±0.010;0.209±0.040 vs 0.721±0.071） （P0.01）.The expressions of TSLC1 and 4.1B showed a significant correlation with cancer differentiation and TNM staging （P0.05）,but not with gender,age and pathological type （P0.05）.The expressions of TSLC1 and 4.1B were positively correlated （r=0.471,P0.001）.Conclusion Down-regulated expressions of TSLC1 and 4.1B in non-small cell lung cancer,both may participate in a cascade of non-small cell lung cancer occurrence and development.TSLC1 and 4.1B are promising targets for non-small cell lung cancer diagnosis and treatment.