3个三角梅品种中类黄酮的毛细管电泳分析比较研究(英文)

Analysis and Comparison of Flavonoids in Three Bougainvilleas by Micellar Electrokinetic Chromatography

甜菜色素和属于类黄酮的花青素在自然界排斥分布,然而我们在甜菜色素代谢类型植物三角梅中发现积累多种类黄酮。为了进一步证实这一现象,本研究对3种白花三角梅（Bougainvillea sp.,Bougainvillea glabra‘A l-ba,’and Bougainvillea‘White Stripe’）中的5种类黄酮（槲皮素、山奈酚、异鼠李素、杨梅素和芹菜素）采用毛细管电泳分析方法进行了调查。结果表明,只有3种类黄酮：槲皮素、山奈酚、异鼠李素被检测出。3个材料中,被探测出来的总黄酮含量最高的是Bougainvillea glabra‘Alba,’1749μg.g-1,Bougainvillea‘White Stripe’1 241μg.g^-1以及B.sp1312μg.g^-1。被探测出来的3种黄酮中,槲皮素含量最高8521 114μg.g^-1,异鼠李素161-628μg.g^-1,而山奈酚之被检测出微量。本研究对于定性定量可食用的三角梅中的类黄酮组成是十分有意义的。甜菜色素和类黄酮在三角梅中的积累关系以及可能的功能本研究也做了讨论。

The exclusion distribution of betalain and flavonoid-derived anthocyanins and existing of flavonoids in betalain-plants led to our research.Quercetin,kaempferol,myricetin,apigenin,and isorhamnetin were simultaneously detected in three white varieties of Bougainvillea（Bougainvillea sp.,Bougainvillea glabra ＇Alba＇,and Bougainvillea ＇White Stripe＇） for the first time,using micellar electrokinetic chromatography（MEKC）.Among the five flavonoids,the three flavonols were detected,and one flavone and one flavonol were not.The total content of the three flavonoids were 1 749 μg·g-1 in B.glabra ＇Alba＇,1 241 μg·g-1 in B.＇White Stripe＇ and 1 312 μg·g-1 in B.sp.In the detected flavonoids,the content of quercetin was the highest and ranged from 852-1 114 μg·g-1,isorhamnetin varied from 161-628 μg·g-1 in three species.Kaempferol was detected at trace amounts.This work is valuable to identify and quantify the naturally occurring flavonoids in the edible bracts of three white varieties of Bougainvillea.The relationship between betalain and flavonoids in bougainvilleas and possible functions were discussed.