人类巨细胞病毒在脐动脉血管平滑肌细胞中的增殖

Multiplication of Human Cytomegalovirus in Umbilical Arteries Vascular Smooth Muscle Cells

目的研究人类巨细胞病毒对血管平滑肌细胞的感染性,为人类巨细胞病毒致动脉粥样硬化及移植物血管病机制研究建立细胞模型。方法自脐动脉分离血管平滑肌细胞,以1个感染复数(MO I)的人类巨细胞病毒感染该细胞,观察细胞病变效应,以RT-PCR技术检测人类巨细胞病毒IE基因在平滑肌细胞内的表达,同时以电镜技术检测平滑肌细胞内的病毒颗粒。结果人类巨细胞病毒感染脐动脉血管平滑肌细胞后第3天即可出现细胞病变,至第8天已出现明显的细胞病变效应,以RT-PCR技术可从感染人类巨细胞病毒AD169株的平滑肌细胞内扩增出预期的产物,经测序验证为人类巨细胞病毒IE基因,以电镜技术可从感染人类巨细胞病毒AD169株的血管平滑肌细胞中观察到病毒颗粒。结论人类巨细胞病毒AD169株可以感染人血管平滑肌细胞,并复制出子代病毒颗粒。为人类巨细胞病毒致动脉粥样硬化及移植物血管病的机制研究提供了较好的实验依据及细胞模型。

Aim To explore the infectivity of human cytomegalovirus（HCMV） in vascular smooth muscle cells and provide a cellular model to the mechanism research of atherosclerosis and transplant vasculopathy induced by this virus. Methods Vascular smooth muscle cells were isolated from umbilical arteries,and HCMV with 1 MOI was co-cultured with these cell,the proliferation of virus in these cells was assured by cytopathic effect,the expression of HCMV IE gene,and virus particles observed by electric microscope. Results Cytopathic effect was observed three days post-infection,and HCMV immediate early（IE） gene was expressed in these cells post-infection by RT-PCR,the correctness was assured by DNA sequencing.With electronic microscope technique,the virus particles were observed in these cells. Conclusion HCMV can infect vascular smooth muscle cells and establish proliferating infection.The work provide a model for the research of mechanisms of atherosclerosis and transplant vasculopathy induced by human cytomegalovrirus.