Lack of association between ADRA2B-4825 gene insertion/deletion poly- morphism and migraine in Chinese Han population

中国汉族人群ADRA2B基因-4825插入/缺失多态与偏头痛易感性无关(英文)

Objective The present study aimed to estimate the association between susceptibility to migraine and the 12nucleotide insertion/deletion （indel） polymorphism in promoter region ofα 2B -adrenergic receptor gene （ADRA2B）.Methods A case-control study was carried out in Chinese Han population,including 368 cases of migraine and 517 controls.Genomic DNA was extracted from blood samples,and DNA fragments containing the site of polymorphism were amplified by PCR.Data were adjusted for sex,age,migraine history and family history,and analyzed using a logistic regression model.Results There was no association between indel polymorphism and migraine,at either the allele or the genotype level.Conclusion These findings do not support a functional significance of ADRA2B indel polymorphism at position-4825 relative to the start codon in the far upstream region of the promoter in the present migraine subjects.

目的研究α2B肾上腺素受体(α2B-adrenergic receptor,ADRA2B)基因启动子区的12碱基插入/缺失多态与偏头痛易感性之间的关系。方法在中国汉族人群中,对368例偏头痛患者和517例健康对照者进行病例对照研究。从受试者外周血中提取基因组DNA,采用PCR法对该多态进行分型。得到的数据采用逻辑回归方法分析,并经过性别、年龄、偏头痛病史及家族史校正。结果在等位基因和基因型水平,该多态均未显示出与偏头痛易感性的相关性。结论从本样本基础上得到的结果并不表明ADRA2B基因启动子区的12碱基插入/缺失多态在偏头痛的发生中起作用。