摘要
在适宜培养条件下，振荡培养４－５ｄ的鸡Ｚｏｎｇ菌和粗粗柄鸡Ｚｏｎｇ菌幼嫩菌丝，经过滤洗涤收信，以０．６ｍｏｌ／ＬＫＣｌ为渗透压稳定剂，用１％纤维素酶加０．５％蜗牛酶的混合酶液处理，于２８℃酶解３－４ｈ，可获得大量原生质体，产量达２．８２＊１０＾６－３．２４＊１０＾６／ｍＬ。但所形的原生质体再生能力较差，再生率仅０．６％－１．２％。本文还详细研究了菌龄，酶系统。
The mycelia shaking- cultured 4-5 days were gathered after being filtered and rinsed. When the mycelia were treated with a combined enzyme system composed of 1 % cellulase and 0.5% glusulase with 0. 6mol/L KC1 being used as osmotic stabilizer at 28℃ for 3-4h, high yield of protoplast (2. 82 - 3. 24×106/mL) was obtained from Termitomyces albuminosus and T. robustus. The protoplast regeneration rate of T. albuminusus and T. robustus was 0.6% - 1. 2% . While the effects of hyphal age, lytic enzyme system, osmotic stabilizer, enzy-molysis temperature and time on the preparation and regeneration of protoplast were also discussed in this paper.
出处
《食用菌学报》
1999年第2期5-9,共5页
Acta Edulis Fungi
基金
贵州省科学基金.项目编号:95096B-138
