针刺对疲劳训练小鼠运动能力的影响

Influence of Acupuncture on the Motor Ability in Training-induced Fatigue Mice

目的:探索针刺改善疲劳训练小鼠运动能力的作用机制。方法:将会游泳的小鼠分为空白对照组、运动对照组、针刺治疗组,每组20只。运动对照组和针刺治疗组进行疲劳训练,疲劳训练的第4周开始对针刺治疗组的小鼠进行针刺治疗,取穴"足三里""关元""肾俞"。治疗2周后,运动对照组和针刺治疗组小鼠做力竭运动,观察小鼠力竭运动时间;然后各组小鼠眼球取血,乳酸氧化酶法检测血清乳酸,免疫抑制法测定血清乳酸脱氢酶,酶偶联法测定血清肌酸激酶。结果:与运动对照组相比,针刺治疗组小鼠的力竭运动时间显著延长(P<0.05)。运动对照组及针刺治疗组的血清乳酸含量均高于空白对照组(P<0.05),运动对照组与针刺治疗组之间的差异无统计学意义(P>0.05)。运动对照组与空白对照组比较,血清乳酸脱氢酶及肌酸激酶均升高(P<0.01);针刺治疗组与运动对照组比较,血清乳酸脱氢酶上升(P<0.05)、血清肌酸激酶下降(P<0.05)。结论:针刺能有效地提高疲劳训练小鼠的运动能力,其机制可能与针刺增加了乳酸脱氢酶的活性和减少了机体微细结构的损伤有关。

Objective To observe the influence of acupuncture on the motor ability of training-induced fatigue mice so as to explore its mechanism underlying acupuncture-induced improvement of physical training fatigue.Methods Sixty Kunming mice were divided into normal control,training model and acupuncture groups,with 20 mice in each.The training-fatigue model was established by forcing the mouse to swim in a water tank for 30 min/d in the 1st week,60 min/d in the 2nd week,90 min/d in the 3rd week and 120 min/d in the 4th and 5th weeks.＂Guanyuan＂（CV 4）,and bilateral ＂Zusanli＂（ST 36） and ＂Shenshu＂（BL 23） were punctured with filiform needles,and with the needle twirled for about 30 s and retained for 5 min.The treatment was conducted once daily for 2 weeks.The duration of exhaustion training was recorded.Serum lactic acid（LA）,lactate dehydrogenase（LDH） and creatine kinase（CK） contents were detected by LA oxidase method,immuno-suppressive assay and enzyme-linked immunoassay,respectively.Results Compared with the training-fatigue（model） group,the duration of the exhausted swimming of the acupuncture group was obviously longer（P0.05）.In comparison with the control group,serum LA,LDH and CK contents were increased considerably in the model and acupuncture groups（P0.05,P0.01）,while compared with the model group,serum LDH level of acupuncture group was increased significantly（P0.05）,and serum CK level of acupuncture group decreased obviously（P0.05）.No significant difference was found between the model and acupuncture groups in serum LA content（P0.05）.Conclusion Acupuncture can effectively improve the motor ability of the training-fatigue mice,which may be closely relevant to its effects in upregulating LDH activity and reducing serum CK.