电针不同单穴与原络配穴对急性心肌缺血家兔心功能及心肌酶的影响

Effects of Electroacupuncture of "Source-" plus "Collateral-" acupoints on the Cardiac Function and Myocardial Enzymes in Acute Myocardial Ischemia Rabbits

目的:探讨电针不同单穴与原络配穴对急性心肌缺血家兔的影响,为临床选穴提供实验依据。方法:随机从健康家兔中选择8只作为正常组,将模型复制成功的家兔随机分为模型组、"神门"组、"内关"组、配穴组,每组8只。"神门"组电针"神门","内关"组电针"内关",配穴组电针"神门""支正"。观察电针治疗后各组家兔心功能变化、血清乳酸脱氢酶(LDH)及心肌肌酸激酶同功酶(CK-MB)含量。结果:"内关"组、"神门"组、配穴组的室内压上升段最大变化率、室内压下降段最大变化率、LDH、CK-MB含量与模型组比较差异有统计学意义(P<0.05,P<0.01),电针"神门"配"支正"穴的改善作用优于"内关"及"神门"穴,而"内关"又优于"神门"穴,差异有统计学意义(P<0.01,P<0.05)。结论:电针原络配穴及不同单穴对急性心肌缺血家兔的心功能及心肌酶含量的影响存在相对特异性,原络配穴对心脏的调整作用优于单个穴位。

Objective To observe the effects of electroacupuncture（EA） of ＂Source-＂＋＂Collateral-＂acupoints [＂Shenmen＂（HT 7）＋＂Zhizheng＂（SI 7）] and single acupoints ＂Neiguan＂（PC 6）and HT 7 on cardiac function and myocardial enzymes in acute myocardial ischemia（AMI）rabbits.Methods Sixty Chinchilla rabbits were randomized into normal control,model,Shenmen（HT 7）,Neiguan（PC 6）,HT 7＋SI 7 groups,with 8 rabbits in each.AMI model was duplicated by occlusion of the anterior descending branch of left coronary artery under anesthesia.EA（2 Hz,1 mA）was applied to acupoints HT 7,PC 6,and HT 7＋SI 7 for 10 min.The maximum ascending rate（＋dp/dt max） and maximum descending rate（-dp/dt max）of the left ventricular pressure（LVP）were recorded by using PowerLab System.Serum lactate dehydrogenase（LDH）and creatine kinase MB（CK-MB）contents were detected with chromatometry.Results Serum LDH and CK-MB contents increased significantly in model group than in normal control group（P0.01）.Compared with model group,serum LDH and CK-MB contents decreased remarkably in PC 6,HT 7 and HT 7＋SI 7 groups（P0.01,P0.05）,and the effects of HT 7＋SI 7 group were significantly superior to those of PC 6 and HT 7 groups in lowering serum LDH and CK-MB levels（P0.01,P0.05）.Compared with the normal control group,＋dp/dt max lowered significantly and-dp/dt max increased markedly in the model group（P0.01）,while compared with the model group,＋dp/dt max increased obviously and-dp/dt max decreased significantly in the three EA groups（P0.01,P0.05）.The effects of HT 7＋SI 7 group were markedly superior to those of HT 7 and PC 6 in raising ＋dp/dt max and lowering-dp/dt max（P0.05,P0.01）,and those effects of PC 6 group were evidently better than those of HT 7 group（P0.05,P0.01）. Conclusion EA of ＂Source-＂＋＂Collateral-＂acupoints（HT 7＋SI 7）,HT 7 and PC 6 has a positive effect in improving changes of cardiac function and in lowering serum LDH and CK-MB contents in AMI rabbits,and the effect of EA of HT 7＋SI 7 is superior to that of EA of single acupoint.