红花多糖对小鼠Lewis肺癌移植瘤生长及转移的影响

Effects of Safflower Polysaccharide on tumor growth and metastasis of mice transplanted with lewis Lung Carcinoma cells

目的研究红花多糖（SPS）对T739小鼠Lewis肺癌移植瘤生长的抑制作用和对肺转移的影响，并初步探讨其作用机制。方法皮下移植Lewis肺癌细胞建立小鼠移植瘤肺转移模型，随机分为4组：对照组、SPS高、中、低剂量组，每日灌胃1次，共28次。测量各组移植瘤体积和重量，镜下观察肺转移灶数目，应用MTT法检测SPS对Lewis肺癌细胞增殖的抑制作用以及对T细胞增殖能力的影响。结果体外SPS对肺癌细胞增殖无抑制作用。体内SPS可促进T淋巴细胞增殖，抑制瘤细胞生长及转移，SPS各剂量组移植瘤体积、重量及肺转移灶数目与对照组比较差异显著有统计学意义（P〈0．05）。结论红花多糖具有抑制肿瘤生长及转移的作用，其作用机制可能与提高自身免疫力有关。

Objective To investigate the inhibitory effects of safflower polysaccharide （SPS） on tumor growth and metastasis of T739 mice transplanted with Lewis lung carcinoma cells, then explore the anti-tumor mechanism of action. Methods The xenografts derived from Lewis lung carcinoma cells were established in T739 mice. Inoculated mice were randomly divided into control group, low dose SPS group, moderate dose SPS group and high dose SPS group, daily oral administration, a total of 28 times. Tumor volume and weight were measured and lung metastatic foci of the mice were observed under microscope. MTF assay was used to investigate both the inhibitive effect of SPS on Lewis lung carcinoma cells and the influence on proliferation of T lymphocytes. Results There was no inhibitory effect of SPS on Lewis lung carcinoma cells in vitro. In vivo, SPS could promote proliferation of T lymphocytes and inhibit tumor growth and metastasis. There were significant differences when comparing the SPS groups with control group in terms of the volume, weight and lung metastatic foci of the transplanted tumor （P 〈 0.05 ）. Conclusion SPS could inhibit the tumor growth and metastasis. The anti-tumormechanism of action was about related to increase the immunological function of mice.