摘要
用pET-21d构建齿兰环斑病毒(ORSV)外壳蛋白基因大肠杆菌表达载体pEO。经SDS-PAGE和Westernblotting分析,含pEO的大肠杆菌正确表达了ORSV外壳蛋白基因。此表达产物为融合蛋白。用表达产物制备抗血清,并应用于间接酶联法检测ORSV,具有较高的灵敏度(10ng/mLORSV)和特异性。
The expression vector pEO in Escherichia coli of the ondontoglossum ringspot virus (ORSV) coat protein gene was constructed by using pET-21d. The ORSV coat protein gene was expressed correctly in E.coli of pEO when tested by SDS-PAGE and Western blotting analysis. The antiserum of the expressed product was prepared by rabbit immunization and was found highly sensitive (10ng/mL ORSV) and specific when used to detect ORSV in indirect-ELISA.
出处
《热带作物学报》
CSCD
1999年第2期62-66,共5页
Chinese Journal of Tropical Crops