直肠腺癌组织TNFRSF7、TNFSF7基因mRNA表达

Expressions of TNFRSF7 and TNFSF7 mRNA in rectal adenocarcinoma

目的检测直肠腺癌组织及癌旁10cm正常组织TNFRSF7、TNFSF7基因表达。方法采集9例直肠腺癌及癌旁10cm正常组织样本,采用原位杂交法检测直肠腺癌组织TNFRSF7、TNFSF7基因表达。将"信号评分"(染色强度评分×染色面积)作为每个样本的原位杂交染色评分。结果 TNFRSF7、TNFSF7生物素标记探针在直肠腺癌细胞中的杂交信号可见于所有直肠腺癌及相应癌旁10cm直肠黏膜上皮。细胞质区域杂交信号的强度较胞核区为弱,TNFRSF7杂交信号的强度较TNFSF7为弱。直肠腺癌组织中TNFRSF7、TNFSF7基因mRNA信号评分均高于癌旁10cm正常黏膜组织。结论 TNFRSF7、TNFSF7mRNA在直肠癌组织中高表达,可作为判断肿瘤分期、指导治疗和预后判断的参考指标。

Objective To detect the expressions of TNFRSF7 and TNFSF7 mRNA in rectal adenocarcinoma and at 10 cm of tumor-adjacent tissures.Methods The expressions of TNFRSF7 and TNFSF7 mRNA in rectal adenocarcinoma（group A）and tumor-adjacen tissures at 10cm apart from the tumor（group B）were performed with in situ hybridization method in 9 patients.The hybridization signal scores（staining intensity staining area）were compared between two groups.Results The hybridization signal of biotinylated probes of TNFRSF7 and TNFSF7 could be detected in all kinds of rectal adenocarcinoma and epithelium mucosa of 10 cm length adjacent to the tumor.The hybridization signal of TNFRSF7 was less than that of TNFSF7.The signal score was higher in group A than that in group B.Conclusion The TNFRSF7 and TNFRSF7 are highly expressed in rectal cancer,which may be taken as the markers for evaluating the tumor stage,directing the treatment and predicting the prognosis of tumor.