胰岛新生相关蛋白对链脲佐菌素诱导的糖尿病小鼠的治疗作用

Efficacy of treating STZ-induced diabetes with INGAP in mice

目的观察腹腔注射胰岛新生相关蛋白(INGAP)对链脲佐菌素(STZ)诱导的糖尿病(DM)小鼠的治疗作用。方法 C57BL/6小鼠腹腔注射STZ建立DM模型,将成模后小鼠随机分为INGAP组和磷酸盐缓冲液(PBS)对照组。INGAP组每日给予腹腔注射INGAP500μg;对照组注射等体积PBS。两组均隔日监测血糖至第34天,用实时荧光定量-PCR检测小鼠胰腺中葡萄糖转运蛋白-2(Glut-2)和胰腺十二指肠同源盒1(PDX-1)mRNA的表达。结果 INGAP组血糖未见明显下降;但与对照组比较,INGAP可以使DM小鼠胰腺中PDX-1、Glut-2mRNA的表达增加(P<0.05)。结论腹腔注射INGAP不能使STZ诱导的DM小鼠血糖下降,但能够使胰腺中的PDX-1、Glut-2表达量提高。

Objective To study the therapeutic effect of islet neogenesis-associated protein（INGAP）on streptozotocin（STZ）-induced diabetes mellitus（DM）.Methods DM models of C57BL/6 mice were established by intraperitoneal injection of STZ.DM mice were randomly divided into two groups of A（daily injected INGAP 500μg）and C（given equivalent volume of saline）for 34 days.Blood glucose was measured every 2 days.The mRNA levels of glucose transporter-2（GLUT-2）and pancreatic duodenal homeodomain-containing protein-1（PDX-1）were detected by RT-PCR on the 34th day.Results Blood glucose was not significcantly decreased in group A.The mRNA expressions of Glut-2 and PDX-1 were all increased in group A than those in group C（P0.05）.ConclusionAlthough PDX-1 and Glut-2 gene expressions in the pancreas are increased in group A,INGAP treatment can not normalize blood glocose in STZ-induced diabetic mice.