IGF-I对体外培养山羊卵泡颗粒细胞增殖与分化的影响

Effects of Insulinlike Growth FactorI on Proliferation and Differentiation of Goat Granulosa Cells In Vitro

为弄清ＩＧＦ－Ｉ在山羊卵泡不同发育阶段对颗粒细胞增殖与分化的影响，分类采集山羊小腔（１～３ｍｍ）和大腔（５～８ｍｍ）卵泡颗粒细胞进行体外培养，对培养后颗粒细胞的ＤＮＡ含量和孕酮分泌量进行测定。结果表明，ＩＧＦ－Ｉ对来源于大、小卵泡颗粒细胞的增殖与分化都有促进作用，对小卵泡颗粒细胞的促增殖作用强，促分化作用弱，而对大卵泡颗粒细胞的促分化作用强，促增殖作用弱。这种差异很可能由大、小卵泡颗粒细胞的增殖与分化活性不同引起。

To study the effects of IGF-I on proliferation and differentiation of granulosa cells during the development of antral follicles in goat, granulosa cells from small antral follicles (1￣3 mm in demeter) and large antral follicles (5￣8 mm in demeter) were cultured in vitro respectively, the granulosa cell DNA content and progesterone secreation were measured at the end of culture. It is showed that, granulosa cells from small antral follicles with an intrinsic high proliferative activity and a low differentiated capbility and granulosa cells from large antral follicles with a restricted proliferative activity and a high differentiated capbility. IGF-I promote both proliferative and differentiated activity in granulosa cells from both large and small antral follicles, but the proliferationpromoting effect in cells from small antral follicles is higher and in cells from large follicles is lower, on the contrary,the differentiationpromoting effect is higher in cells from large follicles and lower in cells from small follicles,this difference effects could be related to difference proliferation and differentiation activity of cells from small and large follicles.