基因芯片筛选应力性骨折差异表达基因

Screening and identification of differential expression genes related to stress fracture by cDNA microarry assay

目的 了解应力性骨折（SF）患者与正常人群相比较的外周血差异表达基因.方法 选取入伍新兵（均为男性）,采用1：1配比的病例对照研究,正常对照组与SF组各3例.收集被测试者空腹肘静脉血提取RNA,纯化后进行人表达谱芯片试验.SAM软件分析芯片结果.选取表达差异最显著并有提示意义的差异基因,应用实时定量聚合酶链反应（PCR）技术进行验证.结果 SF组上调基因21条,下调基因1条.表达差异最显著的基因为TNFRSF10C,其验证结果显示,SF组TNFRSF10C的相对表达量与正常对照组相比较,3组上调,1组下调.结论 SF患者与正常人群外周血之间存在基因表达差异,SF的发生发展是一个涉及多种功能基因表达的复杂的生物学过程,为SF的生物力学研究提供了背景资料,并未其早期诊断和筛查提供了重要线索.

Objective To evaluate the differentially expressed genes between the stress fracture （SF） cases and controls. Methods Total RNA was extracted and purified from peripheral blood sample of 3 SF analysis. The hybridization data were analyzed using SAM software. Parts of these genes were analyzed and identified by real-time PCR. Results Upregulated and downregulated genes were 22 and 1, respectively. Thus the highest ratio and most significant cytokine was tumor necrosis factor receptor superfamily, member 10c （TNFRSF10C）. The result of real-time PCR showed that TNFRSF10C was over-expressed in 3 cases and lowexpressed in 1 case. Conclusion Obvious difference exists in gene expression between SF cases and controls,showing there may be a lot of genes involving in the occurrence and development of SF. Meanwhile, the identification of the specific genes is helpful for biomechanics study, early diagnosis and screening of SF.