鸭疫里默氏杆菌明胶酶对雏鸭免疫功能影响的研究

Effect of gelatinase from Riemerella anatipestifer on immune function in ducklings

为研究鸭疫里默氏杆菌明胶酶对雏鸭免疫功能的影响,本研究将2000 u明胶酶经颈静脉注射10日龄健康鸭建立动物模型,测定了模型动物的部分免疫指标。结果显示,注射明胶酶的雏鸭外周血T淋巴细胞非特异性酯酶阳性率、白细胞杀菌指数、红细胞C3bR花环率、血清溶血和杀菌活性、脾淋巴细胞的增殖和分泌IgG能力等显著低于阴性和空白对照组(p<0.05或p<0.01);明胶酶可导致雏鸭脾脏严重损害,脾中央动脉内皮细胞肿胀、脱落,淋巴细胞变性、坏死及细胞核膜不规则与染色质周边浓集。结果表明,鸭疫里默氏杆菌明胶酶对雏鸭的免疫功能具有损害作用。

To evaluate the effects of gelatinase of Riemerella anatipestifer（RA） on the immune function of duckling,ten-day-old ducklings were injected with gelatinase by jugular vein and the immunological and pathological changes were examined.The acid α-naphthyl acetate esterase（ANAE） positive ratio of the peripheral blood T lymphocytes,bactericidal index of white blood cell（WBC）,the chaplet rate of C3b of red blood cell（RBC）,the hematolysis and bactericidal activity of serum,the generation activity and production IgG of splenic lymphocyte were markedly decreased after injection with gelatinase.Severe damages in spleen,light pathological changes in bursa of fabriciu and thymus were observed under optics and electric-microscope.These results showed that the gelatinase of RA impaired the immune function in ducklings.