COX-2与C/EBP β在人肝细胞肝癌中的表达及其临床意义

Significance of COX-2 and C/EBP β expression in hepatocellular carcinoma

目的:研究COX-2与C/EBP β在肝细胞肝癌(HCC)组织中的表达,二者的相关性及其与临床病理指标的关系.方法:用免疫组织化学方法对59例HCC患者的手术切除标本中COX-2与C/EBP β的表达进行检测.以18例肝血管瘤患者手术切除标本中正常组织作为正常对照.结果:COX-2在HCC组织中呈高表达(阳性率为72.9%,P<0.05);肝功能分级A级,TNM分期Ⅰ、Ⅱ期,肿瘤直径<5cm.在未发生血管侵犯、未发生淋巴结转移以及未发生远处转移的患者中高表达(P<0.05);C/EBP β在HCC中呈高表达(阳性率为47.5%,P<0.05).在有饮酒史、肿瘤直径<5cm、TNM分期Ⅰ、Ⅱ期的HCC患者中高表达;HCC组织中C/EBP β的表达与COX-2呈正相关(r=0.580,P<0.05).结论:COX-2可能与HCC发生的早期阶段有关,C/EBP β相关的信号转导通路可能在肝癌发生早期的COX-2高表达调节中起到一定的作用.

AIM：To investigate the expression of cyclooxygenase-2 （COX-2） and CCAAT/enhancer binding protein β （C/EBP β） and to analyze their correlation with the clinical pathological parameters in hepatocellular carcinoma （HCC）.METHODS：The expression of COX-2 and C/EBP β in 59 HCC tissue specimens and 18 normal hepatic tissue specimens were detected by immunohistochemistry.RESULTS：The positive rate of COX-2 expression in HCC was 72.9% （P0.05）.COX-2 expression was correlated with Child-Pugh classification,tumor diameter,TNM stage,vein invasion,lymph node metastasis,and distant metastasis （P0.05）.The positive rate of C/EBP β expression in HCC was 47.5% （P0.05）.C/EBP β expression was correlated with history of alcohol use,tumor diameter and TNM stage （P0.05）.A close correlation was noted between the expression of COX-2 and C/EBP β （r=0.580,P0.05）.CONCLUSION：COX-2 is overexpressed in HCC and may be involved in early hepatocarcinogenesis.C/EBP β-related signal pathway may upregulate the expression of COX-2 in the early stage of HCC.